One of the main advantages of physical methods, such as size-based techniques, is that they result in label-free, unmodified and viable cells with a simple method, typically resulting in cells that can be used for subsequent downstream methods. Physical separation techniques can also result in a much shorter enrichment time and are expected to cost less without biochemical modifications. However, one of the largest challenges of size-based CTC isolation techniques is the possible interference with other cells, such as leukocytes, that have a size comparable to the lower range of CTC size, while another challenge can occur during validation because of the size difference between CTCs and cancer cell lines, which are regularly used models.
Biochemical Engineering By D G Rao Free Download 12
2ff7e9595c
留言